John null amanda linvill


















The homesteaders, led by Trent Hawkins, pool their resources and determine to fight Susan's ditch. In turn, Susan hires a gunfighter named Leif Crider to "protect" her interests.

These elements set the stage for the story that takes a somewhat unusual course for this era of Gunsmoke. Given the typical Kathleen Hite or John Meston treatment, one would expect an all-out range war to erupt with heavy casualties and no clear "winner.

This episode is fairly formulaic until the last 15 minutes or so. There are a number of story elements introduced near the end that allow for a quick resolution. Crider and his partner Waco hatch a stupid plot to lure Matt and Hawkins to the ranch, but they must be among the worst gunfighters of the time. Matt comes up with a legal solution that introduces a borderline Deus ex machina element to the story and allows for a sudden transformation among the characters.

Christopher Dark's Crider character comes across as more bumbling than evil. Ted Jordan, who would play the recurring character Burke in later seasons, has a brief appearance as Susan's ranch foreman. The story idea behind this episode isn't bad, and it includes a realistic premise for the historical period. However, the execution is lacking. There is never that much tension built between Susan and the homesteaders.

One wonders why Susan is so committed to the cause of building the ditch once she discovers what building the ditch will do to the homesteaders, especially since she seems like a decent enough person. Crider is clearly rotten, and it is a bit absurd that all he has to do is pay Susan a compliment to get her to believe everything he says. Details Edit. Release date October 27, United States. United States. Technical specs Edit.

Runtime 1 hour. Black and White. Contribute to this page Suggest an edit or add missing content. Edit page. See the entire gallery. Supplemental data: Click here to view. Rogers and Yanhong Hao are co—first authors. Capecchi M. Gene targeting in mice: functional analysis of the mammalian genome for the twenty-first century. Welsh, M. Cystic fibrosis. In The metabolic and molecular basis of inherited disease. Scriver, et al. Davis P. Care Med. Schwiebert E. CFTR is a conductance regulator as well as a chloride channel.

Grubb B. Pathophysiology of gene-targeted mouse models for cystic fibrosis. Guilbault C. Cystic fibrosis mouse models. Cell Mol. Choose your target. Wilmut I. Viable offspring derived from fetal and adult mammalian cells. Cibelli J. Cloned transgenic calves produced from nonquiescent fetal fibroblasts. Wakayama T. Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Baguisi A.

Production of goats by somatic cell nuclear transfer. Polejaeva I. Cloned pigs produced by nuclear transfer from adult somatic cells. Chesne P. Cloned rabbits produced by nuclear transfer from adult somatic cells. Zhou Q. Generation of fertile cloned rats by regulating oocyte activation. Lee B. Dogs cloned from adult somatic cells. Cloned ferrets produced by somatic cell nuclear transfer. Sedivy J. Gene targeting and somatic cell genetics — a rebirth or a coming of age?

Trends Genet. Williams S. Evaluation of gene targeting by homologous recombination in ovine somatic cells. Wilmut L. Somatic cell nuclear transfer. Lai L. Production of cloned pigs by using somatic cells as donors.

Cloning Stem Cells. Yanez R. Influence of DNA delivery method on gene targeting frequencies in human cells. Vasquez K. Manipulating the mammalian genome by homologous recombination. Thomas K. High frequency targeting of genes to specific sites in the mammalian genome. Dai Y. Functional disruption of the prion protein gene in cloned goats. Richt J. Production of cattle lacking prion protein.

Kuroiwa Y. Sequential targeting of the genes encoding immunoglobulin-mu and prion protein in cattle. McCreath K. Production of gene-targeted sheep by nuclear transfer from cultured somatic cells.

Robinson N. Some functional consequences of species differences in lung anatomy. McKay K. Zero-stress state of intra- and extraparenchymal airways from human, pig, rabbit, and sheep lung.

Maina J. Morphometric characterization of the airway and vascular systems of the lung of the domestic pig, Sus scrofa : comparisons of the airway, arterial and venous systems. A Mol. Pabst R. The respiratory immune system of pigs.

The immune system of the respiratory tract in pigs. Cooper D. Will the pig solve the transplantation backlog? Zielenski J. Cystic fibrosis: genotypic and phenotypic variations. Dalemans W. Teem J. Receptors Channels. Skach W. Defects in processing and trafficking of the cystic fibrosis transmembrane conductance regulator. Kidney Int. Denning G. Processing of mutant cystic fibrosis transmembrane conductance regulator is temperature-sensitive.

Lukacs G. Pharmacologic approaches to correcting the basic defect in cystic fibrosis. Verkman A. CFTR chloride channel drug discovery — inhibitors as antidiarrheals and activators for therapy of cystic fibrosis. Ostedgaard L. Park K. Production of nuclear transfer-derived swine that express the enhanced green fluorescent protein.

Trapnell B. Expression of the cystic fibrosis transmembrane conductance regulator gene in the respiratory tract of normal individuals and individuals with cystic fibrosis. Colledge W. Zeiher B. Inoue N. High-fidelity correction of mutations at multiple chromosomal positions by adeno-associated virus vectors.

Hirata R. Targeted transgene insertion into human chromosomes by adeno-associated virus vectors. Porteus M. Efficient gene targeting mediated by adeno-associated virus and DNA double-strand breaks. Russell D. Human gene targeting by viral vectors. Hendrie P. Gene targeting with viral vectors. Sun X. Adeno-associated virus—targeted disruption of the CFTR gene in cloned ferrets.

Thomson A. Gene targeting in livestock. Wilschanski M. Mutations in the cystic fibrosis transmembrane regulator gene and in vivo transepithelial potentials. Wilson D. Uncertainty in the diagnosis of cystic fibrosis: possible role of in vivo nasal potential difference measurements. Gelrud A. Analysis of cystic fibrosis gener product CFTR function in patients with pancreas divisum and recurrent acute pancreatitis.

Behm J. Hyposecretion of beta-adrenergically induced sweating in cystic fibrosis heterozygotes. Sato K. Pomp D. Sex identification in mammals with polymerase chain reaction and its use to examine sex effects on diameter of day or pig embryos.

Sambrook, J. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press. Yan Z. Ubiquitination of both adeno-associated virus type 2 and 5 capsid proteins affects the transduction efficiency of recombinant vectors. Generation of cloned transgenic pigs rich in omega-3 fatty acids. Machaty Z. Complete activation of porcine oocytes induced by the sulfhydryl reagent, thimerosal.

In vitro development of preimplantation porcine nuclear transfer embryos cultured in different media and gas atmospheres. Full text links Read article at publisher's site DOI : Smart citations by scite.

The number of the statements may be higher than the number of citations provided by EuropePMC if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles. Explore citation contexts and check if this article has been supported or disputed. Gene therapy for cystic fibrosis: new tools for precision medicine.

Data Data behind the article This data has been text mined from the article, or deposited into data resources. BioStudies: supplemental material and supporting data. Nucleotide Sequences 3. Faculty Opinions. Similar Articles To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation. Adeno-associated virus-targeted disruption of the CFTR gene in cloned ferrets.

Spliceosome-mediated RNA trans-splicing with recombinant adeno-associated virus partially restores cystic fibrosis transmembrane conductance regulator function to polarized human cystic fibrosis airway epithelial cells. Targeted genome editing by recombinant adeno-associated virus rAAV vectors for generating genetically modified pigs.

The porcine lung as a potential model for cystic fibrosis. Funding Funders who supported this work. Howard Hughes Medical Institute. Joining Europe PMC. Tools Tools overview. ORCID article claiming. Journal list. Grant finder. External links service. Annotations submission service. Developers Developer resources. API case studies. Related Stories. Lifestyle Ruth E. Omicron European Shooting Stars Unveiled. All Rights Reserved. Close the menu The Hollywood Reporter homepage.



0コメント

  • 1000 / 1000